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Lima,Matheus Thomaz Nogueira Silva; Santos,Larissa Batista dos; Bastos,Rafael Wesley; Nicoli,Jacques Robert; Takahashi,Jacqueline Aparecida. |
ABSTRACT Major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of Alzheimer's disease have led to searching new control drugs. The present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. For such finality, five filamentous fungal species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were grown or not with DNA methyltransferases inhibitors (procainamide or hydralazine) and/or a histone deacetylase inhibitor... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial activity; Acetylcholinesterase inhibition; Epigenetic modulation; Penicillium sp.; Talaromyces sp.. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100169 |
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Carvalho,Lydston Rodrigues de; Farias,Luiz Macêdo; Nicoli,Jacques Robert; Silva,Maria Clara Fernandes; Corsino,Andrea Teresa Santos Meira; Lima,Luiz André de; Redondo,Rodrigo Aparecido Fernandes; Ferreira,Paulo César Peregrino; Pinto,Maria Elizabeth Bernardes Margutti. |
Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture; Microbiota; Digestive tract; Antagonistic substance. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300007 |
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Duarte,Silvana; Silva,Flávia Cristina de Paula e; Zauli,Danielle Alves Gomes; Nicoli,Jacques Robert; Araújo,Francisco Gerson. |
The Gram-negative intestinal microbiota of Hypostomus auroguttatus and Pimelodus maculatus, a detritivorous and an omnivorous fish species, respectively, were compared between fishes from the reservoir and the stretch of the river below the dam of the Funil hydroelectric plant, Rio de Janeiro, Brazil. Four selective culture media were used under aerobic and two under anaerobic conditions. The omnivorous species had microbiota with higher population levels compared to the detritivorous species. The number of morphotypes and population levels of total bacteria, vibrio and Bacteroides tended to be higher in summer and autumn in the reservoir, and not different in the river. The number of morphotypes of enterobacteria and total bacteria were higher in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alimentary regimen; Environment; Gut microbiology; Hypostomus auroguttatus; Pimelodus maculatus. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400019 |
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Silva,Juliana Fonseca Moreira da; Prado,Guilherme; Madeira,Jovita Eugênia Gazzinelli Cruz; Oliveira,Marize Silva; Faraco,André Augusto Gomes; Malta,Camilla Martins; Nicoli,Jacques Robert; Pimenta,Raphael Sanzio. |
Neste estudo, verificou-se a utilização de um filme de quitosana para o controle do crescimento e produção de aflatoxinas por A. parasiticus em amendoim. Os filmes foram aplicados sobre os grãos por meio de duas metodologias (aspersão e imersão). Os grãos recobertos foram inoculados com 2,5 ml de uma suspensão contendo 1,0×106 esporos/ml e incubados a 25 oC por 7 dias. A concentração de aflatoxinas foi determinada por cromatografia em camada delgada, utilizando a técnica de densitometria. A verificação da inibição do crescimento fúngico foi realizada por meio da inoculação do patógeno em placas de Petri contendo GYEP suplementado com quitosana a 2% e incubadas por 7 dias a 25 ºC. Após incubação, o diâmetro da colônia do patógeno foi estimado e comparado... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis hypogaea; Aspergillus parasiticus; Micotoxinas; Pós-colheita; Substância GRAS. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000400467 |
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